NGS WORKFLOW

Our NGS workflow offers automated library prep which leads to decreased hands-on time and faster turn-around time compared to manual processing. It reduces repeat rates and increases reliability and result reproducibility. The open platform simplifies adaptation to personalized workflows.

Employing state of the art Next-Generation Sequencing (NGS) techniques, the Sentosa® SQ HIV Genotyping Assay calls for variants in HIV positive samples. Vela Diagnostics offers a highly automated workflow from sample extraction to report generation of results to rapidly detect variants in the relevant regions of Protease, Reverse Transcriptase and Integrase genes in samples.

FEATURES AND BENEFITS

• From sample to result in ~ 2 days
• Reliable, reproducible variant calling
• Covers the protease, reverse transcriptase and integrase genes
• Seamless sample ID traceability and IT connectivity
• Automatic data analysis and report generation with Sentosa SQ Reporter
• UDG to eliminate carry-over contamination

Flexibility for virology, combine up to 4 assays (HIV-1, HCV, HBV, CMV) in one NGS run The ViroKey® SQ FLEX Genotyping Assay (4x16) is a next generation sequencing-based test intended for the detection of genomic mutations of the Human Immunodeficiency virus (HIV-1), Hepatitis C virus (HCV), Hepatitis B virus (HBV) and Cytomegalovirus (CMV) that confer resistance to specific types of antiviral drugs from human plasma / serum. Up to 4 tests can be performed in one NGS run. The  ViroKey SQ FLEX Genotyping Assay (4x16) is used in conjunction with the Sentosa® SX Virus Total Nucleic Acid Plus (4x24) as well as the Sentosa SQ 318 Chip Kits, Sentosa ST Template Kits and the Sentosa SQ Sequencing Kits on the Sentosa SX101, Sentosa ST401 and Sentosa SQ301 instruments.